Thermo Fisher Scientific Goat anti-Mouse IgM (Heavy chain) Secondary Antibody, Alexa Fluor 647
Alexa Fluor 647로 결합된 Goat anti-Mouse IgM (Heavy chain) 2차 항체로, WB, IHC, ICC, Flow Cytometry에 적합합니다. Mouse IgM에 특이적으로 반응하며, 높은 순도와 형광 감도를 제공합니다. 연구용으로만 사용됩니다.
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Thermo Fisher Scientific Goat anti-Mouse IgM (Heavy chain) Secondary Antibody, Alexa Fluor 647
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:10,000 |
| Immunohistochemistry (IHC) | 1–10 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–10 µg/mL |
| Flow Cytometry (Flow) | 1–10 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Mouse |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Mouse Mu immunoglobulin |
| Conjugate | Alexa Fluor™ 647 |
| Excitation / Emission Max | 650 / 671 nm |
| Form | Liquid |
| Concentration | 2 mg/mL |
| Purification | Purified |
| Storage Buffer | PBS, pH 7.5 |
| Contains | 5 mM sodium azide |
| Storage Conditions | 4°C, store in dark |
| Shipping Conditions | Ambient |
| RRID | AB_2535807 |
| Target | IgM (Heavy chain) |
| Cross Adsorption | Against human IgG1, IgG2, IgG3, IgG4, IgA, human serum and purified human paraproteins prior to conjugation |
| Antibody Form | Whole Antibody |
Product Specific Information
- Fluorescence of this long-wavelength Alexa Fluor dye is not visible through a conventional fluorescence microscope.
- Product will be shipped at room temperature.
Target Information
Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins. They are useful for detection, sorting, or purification of the specified target.
Secondary antibodies offer increased versatility, enabling use with various detection systems such as HRP, AP, or fluorescence.
They provide greater sensitivity through signal amplification, as multiple secondary antibodies can bind to a single primary antibody.
Typically, these antibodies are generated by immunizing the host animal with pooled immunoglobulins from the target species and further purified or modified (e.g., immunoaffinity chromatography, fragmentation, label conjugation) to yield highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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