Thermo Fisher Scientific CUL4B Monoclonal Antibody (OTI1C4), TrueMAB

Applications and Tested Dilution

Product Specifications

Product Specific Information

For reconstitution, add 100 µL distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).

Target Information

CUL4B is a core component of multiple cullin-RING-based E3 ubiquitin-protein ligase complexes that mediate ubiquitination and proteasomal degradation of target proteins.
It functions as a scaffold protein, positioning substrates and ubiquitin-conjugating enzymes for catalysis.
CUL4B participates in polyubiquitination of CDT1, histones H2A, H3, and H4 in response to DNA damage, and is targeted to UV-damaged chromatin by DDB2, playing a role in DNA repair and replication.
It is required for ubiquitination of cyclin E, ensuring normal G1 cell cycle progression, and regulates the mTOR pathway involved in cell growth and metabolism.
CUL4B’s regulation of the mTORC1-mediated pathway depends on 26S proteasome function and interaction with MLST8.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.