
Thermo Fisher Scientific DMEM, high glucose, HEPES, no phenol red, 500 mL
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Gibco™
DMEM, high glucose, HEPES, no phenol red
DMEM (Dulbecco`s Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells.자세히 알아보기
DMEM (Dulbecco`s Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12. We offer a variety of DMEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool.
This DMEM is modified as follows:
With | Without |
• High Glucose | • Sodium Pyruvate |
• L-glutamine | • Phenol Red |
• HEPES |
The complete formulation is available.
Using DMEM
DMEM is unique from other media as it contains 4 times the concentration of amino acids and vitamins than the original Eagle`s Minimal Essential Medium. DMEM was originally formulated with low glucose (1 g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate. DMEM contains no proteins, lipids, or growth factors. Therefore, DMEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM uses a sodium bicarbonate buffer system (3.7 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.
cGMP manufacturing and quality system
DMEM is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.
본 제품은 냉장/냉동제품으로 반송된 제품은 전량 폐기 처리 되오니 주문 전 상세 내용 다시 한번 확인 부탁드립니다.
사양
세포주HeLa, 293, Cos-7, and PC-12
세포 유형Primary Fibroblasts, Neurons, Glial Cells, HUVECs, Smooth Muscle Cells
분류Animal Origin-free
농도1 X
형태Liquid
제품 유형DMEM (Dulbecco`s Modified Eagle Medium)
Serum LevelStandard Serum Supplementation
유통 기한12 Months From Date of Manufacture
멸균Sterile-filtered
Sterilization MethodSterile-filtered
첨가제 포함High Glucose, Glutamine, HEPES
첨가제 없음No Phenol Red, No Sodium Pyruvate
Glucose 농도4500 mg/L
제조 품질cGMP-compliant under the ISO 13485 standard
제품라인Gibco™
수량500 mL
배송 조건Room Temperature
Unit SizeEach
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