Thermo Fisher Scientific GZMB Monoclonal Antibody (OTI4E6), TrueMAB
Human GZMB 단백질(Granzyme B)을 인식하는 Mouse monoclonal antibody. Western blot, IHC, ICC/IF에 적합. Lyophilized 형태로 제공되며 PBS buffer에 8% trehalose 포함. 세포사멸 연구 및 cytotoxic T/NK cell 관련 연구에 유용.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
| Immunocytochemistry (ICC/IF) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI4E6 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 21–247 of human GZMB produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
Granzyme B (GZMB) is a serine protease found in cytotoxic T cells and NK cell granules. It plays a key role in inducing apoptosis in target cells by cleaving caspase-3 and initiating the caspase cascade. Granzyme B can also activate mitochondrial apoptosis pathways via Bid protein cleavage. It is essential for immune-mediated cytotoxicity and is a marker of activated cytotoxic lymphocytes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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