Thermo Fisher Scientific DUT Polyclonal Antibody
DUT 단백질을 인식하는 Thermo Fisher Scientific의 Rabbit Polyclonal Antibody. Western blot, IHC, ICC, ELISA 등 다양한 응용 가능. 고순도 Protein A 정제, 1 mg/mL 농도, 안정적 TBS/BSA/glycerol buffer 구성. 연구용으로만 사용.
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Thermo Fisher Scientific DUT Polyclonal Antibody
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200 |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| ELISA | 1:500–1:1,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH conjugated synthetic peptide derived from human DUT, amino acids 191–237 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.01M TBS, pH 7.4, with 1% BSA, 50% glycerol |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | −20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
This gene encodes an essential enzyme of nucleotide metabolism. The encoded protein forms a ubiquitous, homotetrameric enzyme that hydrolyzes dUTP to dUMP and pyrophosphate.
This reaction serves two cellular purposes:
- Providing a precursor (dUMP) for the synthesis of thymine nucleotides needed for DNA replication.
- Limiting intracellular pools of dUTP.
Elevated levels of dUTP lead to increased incorporation of uracil into DNA, which induces extensive excision repair mediated by uracil glycosylase. This repair process, resulting in the removal and reincorporation of dUTP, is self-defeating and leads to DNA fragmentation and cell death.
Alternative splicing of this gene leads to different isoforms that localize to either the mitochondrion or nucleus.
A related pseudogene is located on chromosome 19.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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