Thermo Fisher Scientific IDH3A Monoclonal Antibody (OTI2E9), TrueMAB

Applications

Immunocytochemistry (ICC/IF)

• Tested Dilution: 1:100

Flow Cytometry (Flow)

• Tested Dilution: 1:100

Product Specifications

Product Specific Information

For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step.
Recommended desalting product: Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL (Product #89882).

Target Information

Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate.
These enzymes include NAD(+)-dependent and NADP(+)-dependent subclasses.
Five isocitrate dehydrogenases have been identified:

• Three NAD(+)-dependent isocitrate dehydrogenases (mitochondrial matrix)
• Two NADP(+)-dependent isocitrate dehydrogenases (one mitochondrial, one cytosolic)

NAD(+)-dependent isocitrate dehydrogenases catalyze the rate-limiting step of the tricarboxylic acid cycle.
Each isozyme is a heterotetramer composed of two alpha subunits, one beta subunit, and one gamma subunit.
The protein described here is the alpha subunit of one isozyme of NAD(+)-dependent isocitrate dehydrogenase.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.