Thermo Fisher Scientific IDO Polyclonal Antibody
IDO 단백질을 인식하는 Rabbit Polyclonal 항체로, Western blot, IHC, ICC, Flow cytometry, ELISA에 사용 가능. Human, Mouse, Rat, Sheep에 반응하며 Protein A로 정제됨. 연구용으로 면역조절 및 대사 관련 연구에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | Assay-dependent |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:100–1:500 |
| Immunohistochemistry (Frozen) (IHC-F) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
| Flow Cytometry (Flow) | 1:50 |
| ELISA | 1:500–1:1,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Sheep, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH conjugated synthetic peptide derived from human IDO (amino acids 101–200) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.01M TBS, pH 7.4, with 50% glycerol, 1% BSA |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
IDO1 (Indoleamine 2,3-dioxygenase 1) is an intracellular heme-containing enzyme that catalyzes the oxidative cleavage of the indole ring in tryptophan, serotonin, and melatonin. This reaction leads to the production of biologically active metabolites known as kynurenines.
IDO1 is widely expressed in human tissues, macrophages, and dendritic cells. During inflammation, interferons (IFNs) induce IDO1 expression, contributing to antimicrobial defense by degrading tryptophan and inhibiting pathogen growth.
Additionally, IDO1 plays a crucial role in immunoregulation during infection, pregnancy, autoimmunity, transplantation, and cancer.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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