Thermo Scientific™

Phusion™ HF and GC Buffer Packs

5X Phusion Green buffers include a density reagent and two tracking dyes for direct loading of PCR products on a gel.

The error rate of Phusion DNA Polymerase in HF Buffer (4.4 x 10-7) is lower than that in GC Buffer (9.5 x 10-7). Therefore, the HF Buffer should be used as the default buffer for high-fidelity amplification. However, GC Buffer can improve the performance of Phusion DNA Polymerase on some difficult or long templates, such as GC-rich templates or those with complex secondary structures. For applications such as microarray or DHPLC, where the DNA templates need to be free of detergents, we recommend using detergent-free reaction buffers.

사양

설명Phusion™ HF Buffer Pack

중합효소Phusion High-Fidelity DNA Polymerase

제품 유형HF Buffer Pack

수량4 x 1.5 mL

배송 조건Wet or Dry Ice

용도(애플리케이션)Standard PCR, High-fidelity PCR

시작 물질DNA

부피4 x 1.5 mL

색상Colorless

Unit SizeEach