Thermo Fisher Scientific GCP4 Monoclonal Antibody (GT812)
GCP4 단백질을 인식하는 Mouse monoclonal antibody (clone GT812). Western blot 및 ICC/IF에 적합하며, HeLa 세포를 양성 대조군으로 권장. Human에 반응하며, Protein G로 정제된 액상 형태로 제공. 단기 4°C, 장기 -20°C 보관 권장.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:3,000 |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | GT812 |
| Immunogen | Recombinant protein fragment corresponding to amino acids 1–178 of GCP4 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | Store at 4°C (short term). For long-term storage, store at -20°C. Avoid freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2538760 |
Product Specific Information
- Recommended positive control: HeLa cells
- Predicted reactivity: Mouse (97%), Zebrafish (90%), Xenopus laevis (90%), Rabbit (97%), Chimpanzee (100%), Bovine (100%)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
The gamma-Tubulin complex is composed of gamma Tubulin and associated proteins GCP2, GCP3, GCP4, GCP5, and GCP6, which are essential for microtubule organizing centers. These components localize to the centrosome for microtubule nucleation and also exist as soluble complexes in the cytoplasm.
GCP4 (gamma-Tubulin complex component 4, also known as TUBGCP4) is a 667 amino acid protein that localizes to the metaphase spindle during mitosis. Upon proteasome inhibition, GCP4 accumulates at the pericentriolar material, participating in microtubule organization and nucleation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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