Thermo Fisher Scientific p14ARF Polyclonal Antibody
Thermo Fisher Scientific의 p14ARF Polyclonal Antibody는 인간 및 마우스 시료에서 p14ARF 단백질을 검출하는 고품질 항체입니다. Western blot, IHC, ICC 등 다양한 응용에 적합하며, 항원 친화 크로마토그래피로 정제된 액상 제품입니다. 연구용으로만 사용됩니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:100–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:10–1:500 |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:10–1:500 |
| Immunocytochemistry (ICC/IF) | 1:100–1:2,000 |
| Flow Cytometry (Flow) | 1:400 |
| ELISA | 1:100–1:2,000 |
| Immunoprecipitation (IP) | Assay-dependent |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to a portion of human p14ARF (between residues 50–150) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_1959156 |
Product Specific Information
PA1-31409 detects p14ARF from human samples.
Target Information
The INK4a locus on chromosome 9p21 is frequently affected in human tumors. It encodes two structurally distinct tumor suppressor proteins, p16INK4a and the alternative reading frame protein, ARF (p14ARF in human and P19ARF in mouse).
These proteins interact with upstream tumor suppressors, retinoblastoma protein (pRB) and p53, respectively, playing crucial roles in cellular senescence, cell cycle control, and tumor suppression.
p14ARF (or P19ARF in mouse) can induce both G1 and G2 phase arrest in a p53-dependent manner. The functional differences between mouse and human ARF may contribute to species-specific tumor susceptibility.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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