Thermo Fisher Scientific p14ARF Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:100-1:2,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:10-1:500

-

Immunohistochemistry (Frozen) (IHC (F))

1:10-1:500

-

Immunocytochemistry (ICC/IF)

1:100-1:2000

-

Flow Cytometry (Flow)

1:400

-

ELISA (ELISA)

1:100-1:2,000

-

Immunoprecipitation (IP)

Assay-dependent

-

Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to a portion of human p14ARF (between residues 50-150). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:p14ARF,uniProtId:P42771-1,ncbiNodeId:9606,antigenRange:50-150,antigenLength:156,antigenImageFileName:PA1-31409_p14ARF_P42771-1_Rabbit.svg,antigenImageFileNamePDP:PA1-31409_p14ARF_P42771-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1959156

Product Specific Information

PA1-31409 detects p14ARF from human samples.

Target Information

The INK4a locus on chromosome 9p21 is frequently affected in human tumors. It encodes two structurally distinct tumor suppressor proteins, p16INK4a and the alternative reading frame protein, ARF (p14ARF in human and P19ARF in mouse). These two proteins interact with the upstream proteins: retinoblastoma protein and p53 tumor suppressors, respectively. Each of these proteins has a role in the senescence of primary cells, activates pathways for cell cycle control and tumor suppression. The p14ARF (or P19ARF in mouse) proteins can induce both G1 and G2 phase arrest in a manner that depends on functional p53. The mouse and human ARF proteins have different functions in tumor suppression; this distinction may contribute to the different levels of tumor proneness of these species.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.