Thermo Fisher Scientific AFP Recombinant Rabbit Monoclonal Antibody (003)
Human AFP 단백질을 인식하는 Thermo Fisher Scientific의 재조합 토끼 단클론 항체입니다. Western blot, ELISA, IP에 사용 가능하며 높은 특이성과 민감도를 제공합니다. 보존제가 없으며, 동물 유래 성분이 없는 일관된 로트 품질을 보장합니다.
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Thermo Fisher Scientific AFP Recombinant Rabbit Monoclonal Antibody (Clone 003)
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| ELISA | 1:5,000–1:10,000 |
| Immunoprecipitation (IP) | 0.5–2 µL/mg of lysate |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 003 |
| Immunogen | Recombinant Human AFP/alpha-fetoprotein protein (Met1–Val609) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | Store at 4°C (short term); -20°C (long term). Avoid freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2784944 |
Product Specific Information
This product is preservative-free. It is recommended to add sodium azide (final concentration 0.05%–0.1%) to prevent contamination.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems, developed by cloning specific antibody DNA sequences from immunoreactive rabbits. Individual clones are screened for optimal production.
Advantages of recombinant rabbit monoclonal antibodies:
- High specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulation
- Broader immunoreactivity due to large rabbit immune repertoire
This antibody specifically recognizes Human AFP (alpha-fetoprotein).
Target Information
AFP (Alpha Fetoprotein) is a major plasma protein produced by the yolk sac, intestinal tract, and liver during fetal development. In adults, AFP expression is often associated with hepatoma or teratoma, but hereditary persistence may occur without pathology. AFP is considered the fetal counterpart of serum albumin, with both genes located tandemly on chromosome 4.
AFP exists in monomeric, dimeric, and trimeric forms and can bind copper, nickel ions, fatty acids, and bilirubin. AFP levels in amniotic fluid are used to screen for neural tube defects such as spina bifida and anencephaly. Elevated serum AFP is observed in hepatocellular carcinoma, teratoblastoma, colorectal, pancreatic, and germ cell cancers.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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