Thermo Fisher Scientific TRIM25 Monoclonal Antibody (5B5B10)
TRIM25 단백질을 인식하는 Mouse monoclonal antibody로 Western blot, IHC, ICC/IF, Flow cytometry 및 ELISA에 적합. Human 및 Mouse 반응성. Protein G 정제, 1 mg/mL 농도, PBS buffer에 sodium azide 포함. 연구용으로만 사용 가능.
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Applications 및 Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:50–1:250 |
| Flow Cytometry (Flow) | 1:200–1:400 |
| ELISA | 1:10,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 5B5B10 |
| Immunogen | Purified recombinant fragment of human TRIM25 (AA: 211–360) expressed in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2787559 |
Product Specific Information
MA5-31936 has been tested in indirect ELISA.
Target Information
TRIM25, also known as estrogen responsive finger protein (EFP), is a member of the RING finger-B box-coiled coil family and a downstream target of estrogen receptor α. Mice lacking this gene displayed an underdeveloped uterus and reduced estrogen responsiveness, demonstrating that TRIM25 is essential for estrogen-dependent proliferation.
TRIM25 expression is increased in breast carcinoma, promoting tumor growth by functioning as an E3 ubiquitin ligase targeting the negative cell cycle checkpoint 14-3-3σ.
Recent studies indicate that TRIM25 E3 ubiquitin ligase is essential for the retinoic-acid-inducible gene 1 (RIG-1) signaling pathway, contributing to host antiviral innate immunity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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