Thermo Fisher Scientific Asialo GM1 Polyclonal Antibody, Functional Grade, eBioscience

Applications and Publications

Product Specifications

Product Specific Information

This rabbit polyclonal antibody reacts with asialo ganglio-N-tetraosylceramide (asialo-GM1), a glycolipid present on human, mouse, rat, bovine, and hamster cells. Glycolipids are defined by specific transferases that modify the lipid structure and can serve as immune response targets or co-receptors for pathogens.

Asialo-GM1 is found on NK cells and subsets of monocytes/macrophages in mouse spleen, as well as in rat and bovine brain, fetal liver, and immature thymocytes. Expression levels may increase with tumor progression. The antibody specifically recognizes asialo-GM1 and not other glycolipids such as GM1 or asialo-GM2.

This antibody has been reported to deplete NK cells in mice and hamsters in vivo and shows in vitro activity across multiple species. It is suitable for flow cytometric analysis, NK cell depletion, and functional studies. Intravenous or intraperitoneal injection of 20–50 µL can reduce NK1.1 populations after 3–4 days; repeat injections may be required.

Applications Tested:
Validated for flow cytometric analysis of mouse splenocytes. Optimal staining concentration should be determined experimentally.

Endotoxin Level:
< 0.001 ng/µg antibody (LAL assay)

Storage and Handling:
Reconstitute with 1 mL sterile, endotoxin-free water at room temperature. After reconstitution, filter through a 0.22 µm membrane before injection.

Target Information

Asialo ganglio-N-tetraosylceramide (asialo-GM1) is a glycolipid expressed on human, mouse, rat, bovine, and hamster cells. It serves as a target for immune responses and can act as a co-receptor for pathogens. Expression is observed in NK cells, macrophages, brain, fetal liver, and thymocytes, and may increase during tumor progression.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.