ELK Biotechnology Akt1 (phospho Tyr474) rabbit pAb
Human Akt1 단백질의 Tyr474 인산화 부위를 인식하는 rabbit polyclonal antibody로, WB, IHC, IF, ELISA에 사용 가능. 높은 특이성과 민감도로 세포 내 Akt1 활성화 연구에 적합. -20°C에서 1년 안정적 보관 가능.
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제품명
Akt1 (phospho Tyr474) rabbit pAb
제품 개요
ELK Biotechnology에서 제공하는 이 항체는 인간 Akt1 단백질의 Tyr474 인산화 부위를 인식하도록 제작된 rabbit polyclonal antibody입니다. 세포 내 Akt1의 활성화 및 신호전달 경로 분석에 유용합니다.
제품 정보
| 항목 | 내용 |
|---|---|
| Alternative Names | AKT1; PKB; RAC; RAC-alpha serine/threonine-protein kinase; Protein kinase B; PKB alpha; Proto-oncogene c-Akt; RAC-PK-alpha |
| Applications | WB; IHC; IF; ELISA |
| Recommended Dilutions | Western Blot: 1/500 - 1/2000 Immunohistochemistry: 1/100 - 1/300 ELISA: 1/5000 Other applications: Not yet tested |
| Immunogen | Synthesized peptide derived from human Akt around the phosphorylation site of Tyr474 (AA range: 431–480) |
| Host | Rabbit |
| Storage | -20°C / 1 year |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1 mg/ml |
| Observed Band | 56 kD |
| GeneID (Human) | 207 |
| Human Swiss-Prot No | P31749 |
| Species Reactivity | Human; Mouse; Rat; Monkey |
세포 내 위치
Cytoplasm, nucleus, and cell membrane. Nucleus localization occurs after activation by integrin-linked protein kinase 1 (ILK1). Nuclear translocation is enhanced by interaction with TCL1A. Phosphorylation on Tyr-176 by TNK2 results in membrane localization for further phosphorylation on Thr-308 and Ser-473, leading to activation and nuclear translocation. Colocalizes with WDFY2 in intracellular vesicles (PubMed:16792529).
Background
The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved fibroblasts. AKT1 and AKT2 are activated by platelet-derived growth factor via phosphatidylinositol 3-kinase. In the nervous system, AKT1 mediates growth factor-induced neuronal survival by phosphorylating and inactivating apoptotic components. Mutations in AKT1 are associated with Proteus syndrome. Multiple alternatively spliced transcript variants exist for this gene. (RefSeq, Jul 2011)
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