Thermo Fisher Scientific DOK1 Recombinant Rabbit Monoclonal Antibody (JE54-52)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

-

Product Specifications

Species Reactivity

Human, Rat

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

JE54-52

Immunogen

Recombinant protein within Human DOK1 aa 360-481 if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:DOK1,uniProtId:Q99704-1,ncbiNodeId:9606,antigenRange:360-481,antigenLength:481,antigenImageFileName:MA5-36191_DOK1_Q99704-1_Rabbit.svg,antigenImageFileNamePDP:MA5-36191_DOK1_Q99704-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

TBS, pH 7.4, with 0.05% BSA, 40% glycerol

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2890524

Target Information

DOK1 (P62dok, Docking protein 1) is believed to be a mainly cytoplasmic adaptor protein which down-regulates mitogen-activated protein kinase activation, inhibits cell proliferation and transformation, and promotes cell spreading and cell migration. DOK1 is a major substrate for many tyrosine kinases including c-kit, v-abl, vFPS, EGF, and PDGF. Upon phosphorylation by kinases, DOK1 forms a complex with ras GTPase-activating protein. Phosphorylation on tyrosine residues by the insulin receptor kinase results in the negative regulation of the insulin signaling pathway. DOK1 contains a putative pleckstrin homology domain at the amino terminus and ten PXXP SH3 recognition motifs.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.