Thermo Fisher Scientific BRAF Monoclonal Antibody (4F4)
BRAF 단백질을 특이적으로 인식하는 Mouse Monoclonal Antibody (Clone 4F4). Western Blot 및 ELISA에 적합하며, 인간 시료 반응성. 액상 형태로 제공되며 PBS buffer에 보존제 없이 저장. 연구용으로만 사용 가능.
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Applications
Western Blot (WB)
- Tested Dilution: 1–5 µg/mL
ELISA
- Tested Dilution: 0.3 ng/mL
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 4F4 |
| Immunogen | BRAF (NP_004324, 138–231 a.a.) partial recombinant protein with GST tag (MW of GST tag: 26 kDa) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | See Label |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Sequence of this protein is as follows:
FQNPTDVARS NPKSPQKPIV RVFLPNKQRT VVPARCGVTV RDSLKKALMM RGLIPECCAV YRIQDGEKKP IGWDTDISWL TGEELHVEVL ENVP
Target Information
BRAF is involved in the transduction of mitogenic signals from the cell membrane to the nucleus. It is activated by somatic point mutation in human cancer and is thought to play a role in the postsynaptic responses of hippocampal neurons.
This protein regulates the MAP kinase/ERKs signaling pathway, influencing cell division, differentiation, and secretion.
Mutations in B-Raf have been associated with various cancers, including:
- Non-Hodgkin lymphoma
- Colorectal cancer
- Malignant melanoma
- Thyroid carcinoma
- Non-small cell lung carcinoma
- Adenocarcinoma of lung
A pseudogene located on chromosome X has been identified for B-Raf.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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