Thermo Fisher Scientific OxPhos Human WB Antibody Cocktail

Applications

Tested Dilution

Publications

Western Blot (WB)

1:200-1:1,000

View 14 publications 14 publications

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse

Host/Isotype

Mouse / Ig

Class

Cocktail

Type

Antibody

Clone

Cocktail

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.5 mg/mL

Purification

IgG fraction

Storage buffer

HEPES buffered saline

Contains

0.02% sodium azide

Storage conditions

4° C, do not freeze

Shipping conditions

Wet ice

RRID

AB_2533836

Product Specific Information

This product is a cocktail of multiple OxPhos Mouse anti Human antibodies.

C-I-20 (ND6) (clone) 20E9DH10C12 - Bovine Complex I
This is an optimized premixed cocktail suitable for Western Blotting analysis of the relative levels of the 5 OxPhos complexes in mitochondrial preparations from human sources. These clones were chosen because they are against a subunit that is labile when its complex is not assembled. Moreover, the combination is readily resolved in SDS-PAGE when the appropriate gel conditions are used.

C-II-30 (FeS) (clone) 21A11AE7 - Bovine Heart Complex II

C-III-Core 2 (clone) 13G12AF12BB11 - Bovine Heart Complex II, III

C-IV-II (clone) 12C4F12 -Human Complex IV, subunit II

C-V-alpha (clone) 15H4C4 -Bovine Complex V

Target Information

Altered levels of assembly can arise from mutations in individual subunits, mutations in assembly factors for the complex(es), mtDNA depletion or as a result of physiological and or pathological changes e.g. hormone treatment, exercise, diet or oxidative stress.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.