Thermo Fisher Scientific Atg4B Polyclonal Antibody
Rabbit polyclonal antibody targeting human ATG4B protein. Validated for Western blot and immunoprecipitation. Recognizes residues 343–393 of ATG4B. Supplied as liquid at 0.2 mg/mL, stored at 4°C. For research use only.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunoprecipitation (IP) | 2–5 µg/mg lysate |
Product Specifications
| Property | Description |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 343 and 393 of human Autophagy-related Protein 4 Homolog B |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.20 mg/mL |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
The recommended shelf life for this product is 1 year from the date of receipt.
Target Information
Cysteine protease ATG4B is an enzyme encoded by the ATG4B gene. Autophagy is a critical cellular homeostatic process that controls the turnover of damaged organelles and proteins. ATG4B is a deubiquitin-like protease that plays dual roles in the core machinery of autophagy. It cleaves the C-terminal amino acid of ATG8 family proteins (MAP1LC3, GABARAPL1, GABARAPL2, GABARAP) to reveal a C-terminal glycine. Exposure of this glycine is essential for conjugation of ATG8 proteins to phosphatidylethanolamine (PE) and their insertion into membranes, which is necessary for autophagy. ATG4B is mainly expressed in skeletal muscle, brain, heart, liver, and pancreas.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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