Invitrogen™

TaqMan™ Cells-to-CT™ Control Kit

The Ambion™ TaqMan™ Cells-to-CT Control Kit is designed for use with the TaqMan™ Gene Expression Cells-to-CT Kit. The Control Kit자세히 알아보기

The Ambion™ TaqMan™ Cells-to-CT Control Kit is designed for use with the TaqMan™ Gene Expression Cells-to-CT Kit. The Control Kit includes XenoRNA™ Control, a synthetic RNA transcript with a unique sequence that lacks homology to current annotated biological sequences, making it an ideal control for any experiment. The Control Kit also includes a TaqMan™ Gene Expression Assay for the XenoRNA™ Control target, and a TaqMan™ Gene Expression Assay for the highly expressed endogenous control gene β-actin.

Features of the The Ambion™ TaqMan™ Cells-to-CT Control Kit:
• Confirms sufficient cell input
• Monitors the presence of RT or real-time PCR inhibitors
• Controls for sample normalization

Monitor Inhibition and Confirm Sufficient Cell Input
The TaqMan™ Cells-to-CT™ Control Kit is used to assess inhibition and cell input in Cells-to-CT reactions. For example, the XenoRNA™ Control confirms that samples can support RT-PCR and is an indicator of reaction inhibitors. By adding the XenoRNA™ Control to the Stop Solution during the last step of the cell lysis procedure, each sample is provided with a constant amount of control target and subsequently amplified by the XenoRNA™ TaqMan™ Gene Expression Assay.

Amplification with the β-actin Gene Expression Assay functions as an endogenous control for normalization, and ensures that the lysis reaction contains a sufficient number of cells—this is important for experiments with fewer than ∼100 cells and monitors the effectiveness of cell lysis.

Evaluate Compatibility with New Cell Lines
The TaqMan™ Cells-to-CT™ Control Kit is ideal for assessing the compatibility of untested cell lines with the TaqMan™ Gene Expression Cells-to-CT Kit to determine the maximum number of cells that can be used per lysis reaction.

사양

제어 템플릿XenoRNA™ control (synthetic RNA transcript)

검출 방법Primer-probe

형식Tube

GC-Rich PCR PerformanceHigh

PCR 방법qPCR

유전자 기호ACTB

그린 기능Fewer resources used and less waste, Less hazardous

내부 프로브 수정MGB (Minor Groove Binder) (3), Non-Fluorescent Quencher (3), FAM (5`)

라벨 또는 염료FAM

제품라인Ambion™, Cells-to-CT™, TaqMan™

제품 유형Cells-to-C Control Kit

수량100 reactions

배송 조건Dry Ice

충분100 Reactions

타겟XenoRNA™ control, β-actin

용도(애플리케이션)Gene Expression

반응 수100 Reactions

Unit SizeEach