Thermo Fisher Scientific TaqMan Cells-to-CT Control Kit

Invitrogen™ TaqMan™ Cells-to-CT™ Control Kit

The Ambion™ TaqMan™ Cells-to-CT Control Kit is designed for use with the TaqMan™ Gene Expression Cells-to-CT Kit.
This Control Kit includes XenoRNA™ Control, a synthetic RNA transcript with a unique sequence that has no homology to known biological sequences, making it an ideal control for any experiment.
It also contains TaqMan™ Gene Expression Assays for both the XenoRNA™ Control target and the highly expressed endogenous control gene β-actin.

주요 특징

• 충분한 세포 입력량 확인
• RT 또는 실시간 PCR 저해물 존재 여부 모니터링
• 샘플 정규화 제어

Monitor Inhibition and Confirm Sufficient Cell Input

The TaqMan™ Cells-to-CT™ Control Kit is used to assess inhibition and cell input in Cells-to-CT reactions.
The XenoRNA™ Control confirms that samples can support RT-PCR and serves as an indicator of reaction inhibitors.
By adding the XenoRNA™ Control to the Stop Solution during the final step of cell lysis, each sample receives a constant control target amount, which is then amplified using the XenoRNA™ TaqMan™ Gene Expression Assay.

Amplification with the β-actin Gene Expression Assay functions as an endogenous control for normalization and ensures sufficient cell quantity in the lysis reaction.
This is particularly important for experiments involving fewer than approximately 100 cells and helps monitor lysis efficiency.

Evaluate Compatibility with New Cell Lines

The TaqMan™ Cells-to-CT™ Control Kit is ideal for assessing the compatibility of untested cell lines with the TaqMan™ Gene Expression Cells-to-CT Kit, helping determine the maximum number of cells suitable per lysis reaction.

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