Thermo Fisher Scientific Cripto Polyclonal Antibody, Biotin
Cripto 단백질을 인식하는 Biotin 결합 Rabbit Polyclonal 항체. Western blot, ICC/IF, Flow cytometry에 적합. Human 및 Mouse 시료 반응성. 항원 친화 크로마토그래피로 정제되어 높은 특이성과 재현성 제공.
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Applications and Tested Dilution
| Application | Tested Dilution | Notes |
|---|---|---|
| Western Blot (WB) | 2 µg/mL | |
| Immunocytochemistry (ICC/IF) | 1:1,000 | |
| Flow Cytometry (Flow) | Assay-Dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthetic peptide made to an N-terminal portion of the murine Cripto protein sequence (between residues 1–50). |
| Conjugate | Biotin |
| Form | Liquid |
| Concentration | 0.85 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C, store in dark |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_11151971 |
Product Specific Information
In Western blot, a band is seen at approximately 18 kDa representing the core form of Cripto1, and at approximately 36 kDa representing the glycosylated or post-translationally modified form.
Suggested positive control: MDA-MB231 (human breast cancer) lysates.
Target Information
Cripto (TDGF1) is a GPI-anchored cell membrane protein involved in Nodal signaling. Cell-associated TDGF1 acts as a Nodal coreceptor in cis. Shedding of TDGF1 by TMEM8A modulates Nodal signaling by allowing soluble TDGF1 to act as a Nodal coreceptor on other cells. TDGF1 is expressed both in embryonic stem cells and during early embryo development, and is reactivated in a wide range of epithelial cancers in adults.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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