Thermo Fisher Scientific SELK Polyclonal Antibody
SELK 단백질을 인식하는 Thermo Fisher Scientific의 Rabbit Polyclonal Antibody입니다. Western blot 및 Immunocytochemistry에 적합하며 인간 반응성, 높은 순도의 항원 친화 크로마토그래피 정제 제품입니다. 연구용으로만 사용 가능합니다.
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Thermo Fisher Scientific SELK Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:3,000 |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human SELK (exact sequence proprietary) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.48 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 20% glycerol |
| Contains | 0.025% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2736578 |
Product Specific Information
- Positive Control: K562
- Predicted Reactivity: Dog (97%), Pig (91%), Rabbit (89%), Rhesus Monkey (100%), Chimpanzee (100%), Bovine (89%)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
SELK is a selenoprotein containing a selenocysteine residue at its active site. It is localized to the endoplasmic reticulum and highly expressed in the heart, where it may function as an antioxidant. SELK interacts with ER-associated degradation (ERAD) components such as p97 ATPase, Derlin-1 and -2, and SelS, as well as proteins in the oligosaccharyltransferase complex, suggesting involvement in Derlin-dependent ERAD of glycosylated misfolded proteins.
SELK is also implicated in Ca²⁺ flux regulation in immune cells and is cleaved by m-calpain in resting macrophages. This cleavage is inhibited upon activation through various Toll-like receptors.
Usage Notice
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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