Thermo Fisher Scientific Bcl-2 Monoclonal Antibody (100/D5)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:50-1:200

View 41 publications 41 publications

Immunohistochemistry (IHC)

-

View 31 publications 31 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:100

View 4 publications 4 publications

Immunocytochemistry (ICC/IF)

1:10-1:100

View 8 publications 8 publications

Flow Cytometry (Flow)

0.5-1 µg/test

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse, Rat, Rhesus monkey

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

100/D5

Immunogen

A synthetic peptide, aa 41-54 (GAAPAPGIFSSQPG-Cys) of human Bcl-2 protein if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:BCL-2,uniProtId:P10415-1,ncbiNodeId:9606,antigenRange:41-54,antigenLength:239,antigenImageFileName:MA5-11757_BCL-2_P10415-1_House_mouse.svg,antigenImageFileNamePDP:MA5-11757_BCL-2_P10415-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.2 mg/mL

Purification

Protein A/G

Storage buffer

PBS, pH 7.4, with 0.05% BSA

Contains

0.05% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10978135

Product Specific Information

MA5-11757 targets BCL-2 in FACS, ICC/IF, IHC (P), IP, and WB applications and shows reactivity with Human and mouse samples.

The MA5-11757 immunogen is a synthetic peptide, aa 41-54 (GAAPAPGIFSSQPG-Cys) of human Bcl-2 protein.

Target Information

BCL-2 is a key regulator of apoptosis that functions to either inhibit or promote cell death. The BCL-2 family members are also characterized by dimerizing to further modulate apoptosis. Bag1, for example, has been found to form a heterodimer with BCL-2 resulting in the enhancement of the anti-apoptotic effect of BCL-2. Bax and Bak have been shown to play a critical role in cytochrome c release from mitochondria and thus initiate apoptosis. Bax exerts a pro-apoptotic rather than an anti-apoptotic effect on cells. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. In most follicular lymphomas, neoplastic germinal centers express high levels of BCL-2 alpha protein, whereas the normal or hyperplastic germinal centers are negative. Two transcript variants of BCL-2, produced by alternate splicing, differ in their C-terminal ends. The overexpression of BCL-2 has been linked to human cancers such as B-cell lymphoma and prostate cancer.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.