Thermo Fisher Scientific Glutamate-Cysteine Ligase Regulatory Subunit Monoclonal Antibody (CPTC-GCLM-1)

Applications

Western Blot (WB)

• Tested Dilution: 1–2 µg/mL

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: 1–2 µg/mL

Product Specifications

Product Specific Information

• Immunohistochemistry (PFA fixed): Incubate antibody for 30 min at room temperature.
• Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C, followed by cooling at room temperature for 20 minutes.

Target Information

Gamma-glutamylcysteine synthetase (gamma-GCS) is the rate-limiting enzyme for glutathione (L-gamma-glutamyl-L-cysteinylglycine, GSH) synthesis. GSH is ubiquitous in mammalian cells as a vital intra- and extracellular protective antioxidant. Gamma-GCS is a heterodimer composed of a heavy catalytic subunit and a light regulatory subunit that responds to inflammation, phenolic antioxidants, heat shock, oxidants, and cytokines.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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