Thermo Fisher Scientific DDX5 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

0.3-1 µg/mL

-

Immunohistochemistry (Paraffin) (IHC (P))

Assay-dependent

-

Immunocytochemistry (ICC/IF)

1:100

-

RNA Immunoprecipitation (RIP)

2.5 µg/10^6 cells

-

Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Goat / IgG

Class

Polyclonal

Type

Antibody

Immunogen

synthetic peptide PMIGYPMPTGYSQ (aa 602-614). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:DDX5,uniProtId:P17844-1,ncbiNodeId:9606,antigenRange:602-614,antigenLength:614,antigenImageFileName:PA1-31019_DDX5_P17844-1_Goat.svg,antigenImageFileNamePDP:PA1-31019_DDX5_P17844-1_Goat_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

TBS, pH 7.3, with 0.5% BSA

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2230422

Product Specific Information

Recommended positive controls: The peptide used to generate this antibody is available for purchase (GTX10261-PEP)..

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is a RNA-dependent ATPase, and also a proliferation-associated nuclear antigen, specifically reacting with the simian virus 40 tumor antigen. This gene consists of 13 exons, and alternatively spliced transcripts containing several intron sequences have been detected, but no isoforms encoded by these transcripts have been identified.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.