Thermo Fisher Scientific DDX5 Polyclonal Antibody
Western blot, IHC, ICC/IF, RIP 등 다양한 응용에 적합한 DDX5 폴리클로날 항체. 사람과 마우스 반응성. 항원 친화 크로마토그래피로 정제된 고순도 제품. 4°C 단기, -20°C 장기 보관 권장.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.3–1 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent |
| Immunocytochemistry (ICC/IF) | 1:100 |
| RNA Immunoprecipitation (RIP) | 2.5 µg/10⁶ cells |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide PMIGYPMPTGYSQ (aa 602–614) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | TBS, pH 7.3, with 0.5% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2230422 |
Product Specific Information
- Recommended positive controls: The peptide used to generate this antibody is available for purchase (GTX10261-PEP).
- Store product as a concentrated solution.
- Centrifuge briefly prior to opening the vial.
Target Information
DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are involved in various cellular processes that alter RNA secondary structure, including translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Some members of this family are associated with embryogenesis, spermatogenesis, and cell growth/division.
This gene encodes a DEAD box protein that functions as an RNA-dependent ATPase and a proliferation-associated nuclear antigen, specifically reacting with the simian virus 40 tumor antigen. The gene consists of 13 exons, and although alternatively spliced transcripts have been detected, no isoforms have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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