Thermo Fisher Scientific ATG16L1 Polyclonal Antibody
ATG16L1 단백질을 인식하는 염소 유래 폴리클로날 항체로, Western blot 및 IHC(P) 실험에 적합합니다. 인간과 생쥐 시료에 반응하며, 0.5 mg/mL 농도의 액상 형태로 제공됩니다. 자가포식 관련 연구 및 ATG16L1 단백질 검출에 유용합니다.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.01–0.1 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 3.75 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide with sequence C-KVEKVLSKQHSSSIN |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Ammonium sulfate precipitation |
| Storage buffer | TBS, pH 7.3, with 0.5% BSA |
| Contains | 0.02% sodium azide |
| Storage conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Wet ice |
| RRID | AB_2932931 |
Product Specific Information
This antibody is expected to recognize both reported isoforms (NP_110430.5 and NP_060444.3).
Tested in Peptide ELISA: antibody detection limit dilution 1:32,000.
Target Information
Autophagy related protein 16 L (ATG16L1) is encoded by the ATG16L gene located on chromosome 2 in humans. It is also known as WD30 or IBD10 (Inflammatory Bowel Disease 10).
ATG12 and ATG5 conjugate and oligomerize with ATG16L1 to form an ~800 kDa complex (Atg16L complex), which facilitates coupling (lipidation) of LC3 to phosphatidylethanolamine (PE). This ubiquitin-like conjugation system is a key marker for autophagy.
The ATG16L complex, along with other ATGs such as ATG18 and ATG21, prevents premature cleavage of ATG8-PE by the cysteine protease Atg4 until autophagosome completion.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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