Thermo Fisher Scientific LCK Polyclonal Antibody

Thermo Fisher Scientific LCK Polyclonal Antibody

Applications

• Western Blot (WB): 1–3 µg/mL
• Peptide ELISA: Detection limit dilution 1:16,000

Product Specifications

Product Specific Information

• This antibody is not expected to cross-react with other tyrosine protein kinases.
• Tested in Peptide ELISA with antibody detection limit dilution 1:16,000.

Target Information

LCK is a member of the Src-family tyrosine kinases essential for T-cell receptor (TCR) signaling. Upon TCR activation, LCK phosphorylates ITAM motifs on zeta subunits, creating binding sites for ZAP70, which is subsequently phosphorylated and activated by LCK. This signaling cascade leads to LAT phosphorylation and downstream activation.
LCK is mainly localized to the plasma membrane but also found in the Golgi apparatus, potentially contributing to Raf activation under weak TCR stimulation.
It contains N-terminal myristylation and palmitylation sites, a PTK domain, and SH2/SH3 domains that mediate protein-protein interactions.
LCK regulates apoptosis induced by various stimuli, excluding death receptor pathways. Diseases associated with LCK include Immunodeficiency 22 and CD45 deficiency. Alternative splice variants encoding different isoforms have been identified.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.