Thermo Fisher Scientific Pierce FITC Antibody Labeling Kit

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항체 및 단백질을 형광 탐침으로 손쉽게 라벨링할 수 있는 FITC 키트. 1시간 내 라벨링 및 정제 가능. 아민 특이적 반응으로 높은 효율과 안정적 결합 제공. 정제 수지와 스핀 컬럼 포함으로 빠르고 깨끗한 결과 보장.

카탈로그번호: 53027
브랜드: Thermo Fisher Scientific
카테고리: Protein Linking and Labeling Systems
판매단위: pk

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Thermo Fisher Scientific 53027 Pierce FITC Antibody Labeling Kit Each pk판매 단위 pk ·

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Thermo Fisher Scientific · Thermo Fisher Scientific Pierce FITC Antibody Labeling Kit

Thermo Fisher Scientific Pierce™ FITC Antibody Labeling Kit

The Thermo Scientific™ Pierce™ FITC Antibody Labeling Kit is a multi-component kit containing a high-performance derivative of fluorescein dye, activated for easy and reliable labeling of antibodies, proteins, and other molecules for use as fluorescent probes.

Features

Easy: Convenient kit with FITC to label and purify antibody in about one hour
Amine-specific labeling: Isothiocyanate fluorescein efficiently labels antibodies and purified proteins at primary amines (lysine side chains)
Optimized procedure: Produces antibodies with excellent dye-to-protein ratios for optimal fluorescence
Single-use reagents: Includes premeasured vials for convenience
Efficient purification: Purification resin and spin columns enable rapid removal of unreacted dye and high protein recovery

Properties of FITC

항목	내용
Alternative names	5/6-FITC
Chemical name	5(6)-Fluorescein isothiocyanate mixed isomer
Molecular weight	389.2
Excitation source	488 nm spectral line, argon-ion laser
Excitation wavelength	494 nm
Emission wavelength	518 nm
Extinction coefficient	> 70,000 M⁻¹cm⁻¹
CAS #	27072-45-3
Purity	> 95% (HPLC)
Solubility	Soluble in aqueous buffers at pH > 6
Reactive groups	Isothiocyanate (reacts with primary amines at pH 7.0–9.0)

Applications

Label antibodies for immunofluorescent probes
Label oligonucleotides for hybridization probes
Detect proteins in gels and on Western blots

Amine-reactive Fluorescein Dyes

FITC is the fluorescein molecule functionalized with an isothiocyanate (—N=C=S) reactive group. It reacts with primary amines on proteins, peptides, and other biomolecules.
NHS-fluorescein, an alternative derivative, has greater specificity toward primary amines and forms more stable linkages. Pierce amine-reactive fluorescein dyes are mixed isomers (5- and 6-positions) with indistinguishable excitation and emission spectra, suitable for protein labeling without isomer separation.

Specifications

항목	내용
설명	Pierce FITC Antibody Labeling Kit
제품 유형	Labeling Kit
수량	3 reactions kit
용량	Labeling and purifying 3 × 1 mg (2 mg/mL) of IgG or other proteins
Excitation/Emission	494 / 518 nm
라벨링 스케일	0.5 to 1 mg
제품 라인	Pierce™
용해도	Aqueous buffer
라벨 또는 염료	FITC (Fluorescein)
Labeling Target	Antibodies, Proteins
Unit Size	Each