Thermo Fisher Scientific P4HTM Polyclonal Antibody
Thermo Fisher Scientific의 P4HTM Polyclonal Antibody는 인간 PH-4 단백질을 표적으로 하는 토끼 유래 다클론 항체입니다. Western blot에 적합하며, 높은 종간 반응성을 보입니다. PBS 기반 용액 형태로 제공되며, 장기 보관 시 -20°C에서 보관 가능합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.2–1 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide directed towards the middle region of human PH-4 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS with 2% sucrose |
| Contains | 0.09% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2884630 |
Product Specific Information
- Immunogen sequence: RLGNGWWMTP ESIQEMYAAI KADPDGDGVL SLQEFSNMDL RDFHKYMRSH
- Storage guidance:
- Short-term: 2–8°C, up to 1 week
- Long-term: -20°C, store in small aliquots to prevent freeze-thaw cycles
- Predicted homology:
Cow: 100%; Dog: 100%; Guinea Pig: 93%; Horse: 93%; Human: 100%; Mouse: 100%; Pig: 100%; Rabbit: 100%; Rat: 100%
Target Information
Prolyl hydroxylase 4 (P4HTM) is an enzyme that modifies HIF-alpha, a key transcriptional complex involved in oxygen homeostasis. Unlike collagen-modifying prolyl hydroxylases found in the endoplasmic reticulum, P4HTM acts intracellularly on HIF-alpha. Under normoxic conditions, it hydroxylates HIF-alpha, leading to its degradation via the von Hippel-Lindau ubiquitylation complex. Under hypoxic conditions, reduced hydroxylation stabilizes HIF, promoting angiogenesis. P4HTM thus functions as a cellular oxygen sensor and regulator of HIF stability.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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