Thermo Scientific™

First Strand cDNA Synthesis Kit

Thermo Scientific First Strand cDNA Synthesis kit utilizes the recombinant M-MuLV Reverse Transcriptase which exhibits lower RNase H activity than자세히 알아보기

Thermo Scientific First Strand cDNA Synthesis kit utilizes the recombinant M-MuLV Reverse Transcriptase which exhibits lower RNase H activity than AMV reverse transcriptase. Due to this feature, full-length cDNA can be synthesized from RNA templates up to 9 kb. The recombinant RiboLock RNase Inhibitor, supplied with the kit effectively protects RNA template from degradation.

The first strand of cDNA can be directly used as a template in PCR or in second strand cDNA synthesis.

Highlights
• Efficient synthesis of first strand cDNA up to 9 kb
• Reaction temperature 37°C
• Supplied with the recombinant RiboLock RNase Inhibitor
• Complete—oligo(dT)18 and random hexamer primers included with the kit

Applications
• First strand cDNA synthesis for RT-PCR and real-time RT-PCR
• Construction of cDNA libraries
• Generation of probes for hybridization
• aRNA synthesis

The kit is supplied with both oligo(dT)18 and random hexamer primers. The oligo(dT)18 anneals selectively on the poly(A) tail of mRNA. Random hexamer primers do not require the presence of poly(A). Therefore, they can be used for transcription of the 5`-end regions of mRNA or cDNA synthesis using RNA without poly(A) tail e.g. micro RNAs. Gene-specific primers may also be used with the kits.

사양

형식Kit

반응 속도60 min.

기술Reverse Transcription

최적 반응 온도37°C

수량100 reactions

역전사 효소M-MLV

리보뉴클레아제 H 활성Yes

배송 조건Dry Ice

용도(애플리케이션)Real Time PCR (qPCR), RT-PCR

최종 제품 유형First-Strand cDNA

반응 수100 Reactions

반응 형식Separate components

시약 유형Reverse Transcription

크기(최종 제품)Up to 13 kb

시작 물질RNA

Unit SizeEach