Thermo Fisher Scientific PCNA Monoclonal Antibody (PC10 (3F81)), eFluor 615, eBioscience

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

Assay-Dependent

-

Immunocytochemistry (ICC/IF)

0.5 µg/mL

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Mouse / IgG2a, kappa

Recommended Isotype Control

Mouse IgG2a kappa Isotype Control (eBM2a), eFluor™ 615, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

PC10 (3F81)

Conjugate

eFluor™ 615 eFluor™ 615 eFluor™ 615

View additional formats

• Unconjugated
• PE
• Request custom conjugation

Excitation/Emission Max

595/614 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10806972

Product Specific Information

Description: The PC10 antibody recognizes the proliferating cell nuclear antigen (PCNA), a 36 kDa protein, also known as polymerase delta auxiliary protein. PC10 antibody reacts with human, mouse, and rat PCNA. The peak expression of PCNA occurs during the S-phase.

Applications Reported: This PC10 (a.k.a. 3F81) antibody has been reported for use in immunohistochemical and immunocytochemical staining.

Applications Tested: This PC10 (a.k.a. 3F81) antibody has been tested by immunocytochemistry of methanol-fixed MDCK cells and can be used at less than or equal to 0.5 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. This product has not been validated for flow cytometric analysis.

Filter Recommendation: When using this eFluor™ 615 antibody conjugate, we recommend a filter that will capture the 615 emission wavelength (for example, Excitation 560/55, 585LP, Emission 645/75). A standard Alexa Fluor™ 594 filter is acceptable.

Excitation: 595 nm; Emission: 615 nm.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

PCNA (polymerase delta auxiliary protein) is essential for DNA replication and is involved in DNA excision and mismatch repair pathways. PCNA binds to the CDK inhibitor p21, the structure-specific endonucleases Fen1 and XPG, and DNA cytosine 5-methyltransferase (MCMT). PCNA is a potentially useful marker of cells with proliferative potential and for identifying the proliferation status of tumor tissue (i.e. relevant to prognosis). PCNA is a marker for cells in early G1 phase and S phase of the cell cycle. PCNA is found in the nucleus and is a cofactor of DNA polymerase delta, and acts as a homotrimer and helps increase the processing of leading strand synthesis during DNA replication. In response to DNA damage, PCNA is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for PCNA. Pseudogenes of PCNA have been described on chromosome 4 and on the X chromosome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.