Thermo Fisher Scientific MED10 Polyclonal Antibody
Western blot, IHC, ICC/IF에 적합한 Rabbit Polyclonal Antibody. 인간, 마우스, 랫트 반응성. Affinity chromatography로 정제된 1 mg/mL 액상 항체. MED10 단백질의 E10-D60 펩타이드 유래 면역원 사용. 연구용으로만 사용 가능.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human MED10 (Accession Q9BTT4), corresponding to amino acid residues E10–D60 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2850115 |
Product Specific Information
Antibody detects endogenous levels of total MED10.
Target Information
MED10 is a component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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