Thermo Fisher Scientific ECSIT Polyclonal Antibody
ECSIT 단백질을 인식하는 Thermo Fisher Scientific의 Rabbit Polyclonal 항체. Western blot, IHC, ICC/IF에 적합하며 인간, 마우스, 랫트 반응성. 항원 친화 크로마토그래피로 정제된 액상 항체로, NF-κB 활성화 및 TLR 신호 연구에 활용 가능.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.04–0.4 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:500 |
| Immunocytochemistry (ICC/IF) | 0.25–2 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host/Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein corresponding to Human ECSIT. Recombinant protein control fragment (Product #RP-99074). |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.3 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 40% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2640879 |
Product Specific Information
Immunogen sequence:
HMEPDLSARV TIYQVPLPKD STGAADPPQP HIVGIQSPDQ QAALARHNPA RPVFVEGPFS LWLRNKCVYY HILRADLLPP EEREVEETPE EWNLYYPM
Highest antigen sequence identity to the following orthologs:
- Mouse: 84%
- Rat: 85%
Target Information
Activation of NF-kappa-B as a result of Toll-like receptor (TLR) and IL-1 receptor signaling is a major component of innate immune responses. Signals from these receptors are relayed by adapter molecules such as TRIF, TIRAP, and MyD88 to kinases like IRAK and intermediates such as TRAF6.
ECSIT (Evolutionarily Conserved Signaling Intermediate in Toll pathways) was initially identified as a cytoplasmic protein interacting specifically with TRAF6 in the TLR pathway. It is also required for bone morphogenetic protein (Bmp) signaling and mesoderm formation during mouse embryogenesis, suggesting cross-talk between the TLR/IL-B and Bmp signaling pathways.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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