Thermo Fisher Scientific Actin Muscle Monoclonal Antibody (HHF35)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:100-1:500

View 1 publication 1 publication

Immunohistochemistry (IHC)

-

View 10 publications 10 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:100

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:10-1:100

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

HHF35

Immunogen

SDS extract of human myocardium if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Actin Muscle,uniProtId:P62736-1,ncbiNodeId:9606,antigenRange:1-377,antigenLength:377,antigenImageFileName:MA5-14084_Actin_Muscle_P62736-1_House_mouse.svg,antigenImageFileNamePDP:MA5-14084_Actin_Muscle_P62736-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

tissue culture supernatant

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Wet ice

RRID

AB_10975398

Product Specific Information

This antibody reacts with actin from tissue extracts of uterus, ileum, aorta, diaphragm, heart and smooth muscle cells. It recognizes alpha-actin from skeletal, cardiac, and smooth muscle, and reacts with gamma actin from smooth muscle. This antibody will react with tumors from smooth muscle (leiomyomas and leiomyosarcomas) as well as skeletal muscle tumors (rhabdomyomas and rhabdomyosarcomas).

Staining of formalin-fixed tissues requires boiling in 1mM EDTA, pH 8.0, followed by cooling at room temperature for 20 min.

Target Information

The product encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Mutations in this gene cause nemaline myopathy type 3, congenital myopathy with excess of thin myofilaments, congenital myopathy with cores, and congenital myopathy with fiber-type disproportion, diseases that lead to muscle fiber defects.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.