Thermo Fisher Scientific CTNNA3 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein fragment corresponding to a region within amino acids 43 and 319 of Human CTNNA3 if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:CTNNA3,uniProtId:Q9UI47-1,ncbiNodeId:9606,antigenRange:43-319,antigenLength:895,antigenImageFileName:PA5-30535_CTNNA3_Q9UI47-1_Rabbit.svg,antigenImageFileNamePDP:PA5-30535_CTNNA3_Q9UI47-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

0.1M tris glycine, pH 7, with 20% glycerol

Contains

0.01% thimerosal

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2548009

Product Specific Information

Recommended positive controls: HeLa, HepG2, HCT116.

Predicted reactivity: Human (99%), Mouse (92%), Bovine (94%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

Catenins are proteins that connect cadherin/beta-catenin cell adhesion complexes to the actin cytoskeleton. Although originally discovered in testis, alphaT-catenin is expressed in other tissues, the highest levels being observed in heart. Immunohistochemical analysis showed human alphaTcatenin localization at intercalated discs of cardiomyocytes and in peritubular myoid cells of testis. It is proven that alphaT-catenin can functionally restore cell-cell adhesion in colon cancer cells lacking alpha-catenins. This indicates that this protein is involved in the formation of specific cell-cell contacts in specific types of muscle cells. The encoded protein has about the same predicted size (100 kDa) as other alpha-catenins to which it shows an overall amino acid identity of 57%. In cells transfected with alphaT-catenin cDNA, interaction with alpha-catenin was demonstrated by coimmunoprecipitation. The 892_24D2S antibody against alphaT-catenin does not show any cross reaction with alphaE-catenin or alphaNcatenin in western blot analysis.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.