Thermo Fisher Scientific Sanger Sequencing Kit

Applied Biosystems™ Sanger Sequencing Kit

The Applied Biosystems Sanger Sequencing Kit includes ExoSAP-IT Express reagent, BigDye Terminator v3.1 Cycle Sequencing Kit, and BigDye XTerminator Purification Kit, providing all the reagents needed to clean up PCR products, perform cycle sequencing, and clean up sequencing products.
This simple, fast workflow enables completion of Sanger sequencing, from sample to answer, in less than a workday.

ExoSAP-IT Express Reagent: Fastest PCR Cleanup Method

In only 5 minutes, ExoSAP-IT Express PCR Product Cleanup Reagent removes excess primers and unincorporated nucleotides from a PCR reaction.
Eliminate spin columns, magnetic beads, sedimentation, filtration, and gel purification.
The novel ExoSAP-IT technology allows for a significant reduction in sample cleanup time with minimal steps, providing the simplest workflow.

• 5-min protocol
• One-tube, one-step PCR cleanup
• Novel enzyme technology
• 100% recovery of PCR products

BigDye Terminator v3.1 Cycle Sequencing Kit

The BigDye Terminator v3.1 Cycle Sequencing Kit provides premixed reagents for Sanger sequencing reactions.
Its robust, highly flexible chemistry is ideal for de novo sequencing, resequencing, and finishing with PCR product, plasmid, fosmid, and BAC templates.

• Improved quality for a wide range of sequencing applications
• Optimized for long read lengths
• Better dye mobility characteristics
• Improved performance through GT-rich regions
• Longer, higher-quality reads with uniform peak heights and optimal signal balance
• Enhanced productivity and reduced costs

BigDye XTerminator Purification Kit

The BigDye XTerminator Purification Kit is a fast, simple purification method for DNA sequencing reactions that removes unincorporated BigDye terminators and salts.
Cleanup is complete in under 40 minutes and requires less than 10 minutes of labor.

• Eliminate liquid transfer—use a single plate
• Complete dye blob removal
• Stabilize samples before analysis
• Improve sequencing workflow
• Perform manual or automated protocols
• Rapid, reliable, and reproducible results

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