Invitrogen™

ElectroMAX™ DH10B T1 Phage-Resistant Competent Cells

ElectroMAX DH10B T1 Phage-Resistant Competent Cells offer transformation efficiencies that are among the highest we offer and can exceed 1자세히 알아보기

ElectroMAX DH10B T1 Phage-Resistant Competent Cells offer transformation efficiencies that are among the highest we offer and can exceed 1 x 1010 cfu/μg plasmid DNA in efficiency. They are ideal for applications requiring high-efficiency transformation, including cDNA and gDNA library construction.

ElectroMAX DH10B cells:
• Maximize yield of transformants from limited cloning products
• Permit efficient cloning of methylated DNA
• Are resistant to T1 and T5 bacteriophages
• Support blue/white screening by α-complementation on plates containing X-Gal or Bluo-Gal
• Deliver high-yield plasmid preparations for downstream applications

Phage-resistant representative libraries from a single transformation
The ton_A mutation in DH10B cells confers resistance to bacteriophages T1 and T5, safeguarding valuable libraries against contamination. Additionally, mutations in the methylation-dependent restriction system (_mcr_A, _mcr_BC, and _mrr) make ElectroMAX DH10B T1 Phage-Resistant Cells ideal for construction of genomic libraries of both prokaryotic and eukaryotic genomic DNA and allow for efficient plasmid rescue from eukaryotic genomes. ElectroMAX DH10B T1 Phage-Resistant Cells are suitable for construction of gene banks, for the generation of cDNA libraries using plasmid-derived vectors, and for situations when DNA is limiting. This strain also has the Φ80_lac_ZΔM15 genotype, providing for the option of blue/white screening on plates containing either X-Gal or Bluo-Gal. Finally, the _end_A1 mutation makes DH10B an excellent vehicle for amplifying plasmid DNA for subsequent extraction and purification.

Genotype
F-mcr_A Δ(_mrr_-_hsd_RMS-_mcr_BC) Φ80_lac_ZΔM15 Δ_lac_X74 _rec_A1 _end_A1 _ara_D139Δ(_ara, leu)7697 _gal_U _gal_K λ-_rps_L _nup_G _ton_A

Find the strain and format that fits your needs
DH10B cells are available in both electrocompetent and chemically competent formats.
ElectroMAX DH10B cells are suitable for cDNA or gDNA library construction but are not resistant to T1 and T5 phage infection.
MegaX DH10B T1R Electrocomp Cells have the highest transformation efficiency (>3 x 1010 cfu/μg plasmid DNA) and also offer the benefit of T1 and T5 phage resistance.

사양

제품 유형Electrocompetent Cells

에프에피솜 포함No

플라스미드 품질 개선Yes (endA1)

메틸화 DNA 클로닝Yes (mcrA)

형질전환 효율 수준High Efficiency (>1 x 109 cfu/μg)

항생제 내성 박테리아Yes (Streptomycin)

불안정 DNA 클로닝Not suitable for cloning unstable DNA

블루/화이트 스크리닝Yes (lacZΔM15)

고처리량 호환성Low

플라스미드May be used for plasmids >20 kb

비메틸화 DNA 준비No

재조합 감소Yes (recA1)

배송 조건Dry Ice

T1 Phage - 저항성(tonA)Yes

종E. coli (K12)

형식Tube

제품라인ElectroMAX™

수량5 x 100 μL

Unit SizeEach