Thermo Fisher Scientific IL-27 p28 Polyclonal Antibody
Human IL-27 p28을 인식하는 Rabbit Polyclonal Antibody로, WB와 ELISA에 적합. Recombinant Human IL-27 단백질로부터 유래하며, 비보존액 형태로 제공. Protein A 및 항원 친화 크로마토그래피로 정제되어 높은 특이성과 재현성을 보장.
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Applications
Western Blot (WB)
- Tested Dilution: 1:500–1:1,000
ELISA
- Tested Dilution: 1:25,000–1:50,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant Human IL-27 Protein (Met1–Lys229 & Phe29–Pro243) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A, Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | Store at 4°C short term. For long-term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2788010 |
Product Specific Information
This product is preservative-free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%–0.1%).
This antibody has specificity for Human IL-27.
Target Information
IL-27, a member of the IL-12 family, is a heterodimeric protein consisting of the p40-related protein Epstein-Barr virus-induced gene 3 (EBI3) non-covalently linked to an IL-12p35-related protein, p28 (also known as IL-30).
IL-27 is produced by activated APCs and mature dendritic cells and exerts its activities on NK cells and naive CD4+ T cells.
It activates JAK1, STAT1, and STAT3 pathways via WSX-1/gp130 binding.
Deficiency in WSX-1/TCCR leads to impaired Th1 responses, indicating IL-27’s crucial role in Th1 initiation.
IL-27 induces T-bet expression and suppresses GATA-3, contributing to Th1 differentiation.
Recent studies show IL-27 exhibits potent antitumor activity by enhancing CTL function and inhibiting tumor growth.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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