Thermo Fisher Scientific H2AX Polyclonal Antibody

Applications and Tested Dilution

Product Specifications

Product Specific Information

• Recommended shelf life: 1 year from date of receipt
• Application Note: For IHC, epitope retrieval with citrate buffer (pH 6.0) is recommended for FFPE tissue sections.

Target Information

Histone H2A.X (H2AX) is a member of the histone H2A family, one of the four core histones forming the nucleosome core particle. In eukaryotes, DNA double-strand breaks (DSBs) trigger phosphorylation of serine 139 at the C-terminus of histone H2AX, resulting in γ-H2AX formation. This phosphorylation can be detected by Western blotting or immunofluorescence to assess DSB frequency.

Phosphorylation of H2AX is mediated by phosphatidylinositol 3-kinases, including ATM, DNA-PK, and ATR. H2A.X contains 143 amino acids and is synthesized both in G1 and S-phase. Two forms of its mRNA exist: a short replication-type (575 bases, lacking polyA) and a longer basal-type (1600 bases, containing polyA). The gene maps to human chromosome 11q23.2–q23.3. H2A.X plays a crucial role in chromatin structure and DNA repair signaling via MDC1 recruitment to DNA damage sites.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.