Thermo Fisher Scientific Pierce Fluorescent Protease Assay Kit

Thermo Scientific™ Pierce™ Fluorescent Protease Assay Kit

The Thermo Scientific Pierce Fluorescent Protease Assay Kit measures total protease activity in samples, providing a means to assess the progress of protease isolation procedures or quantify protease contamination in biological samples.

This kit includes fluorescein-labeled casein (FITC-casein) as a substrate for assessing protease activity by either fluorescence resonance energy transfer (FRET) with a standard fluorometer or fluorescence polarization (FP) with capable instrumentation. FTC-Casein is native casein labeled using a large molar excess of fluorescein isothiocyanate (FITC). The fluorescence properties of this heavily labeled, intact protein substrate change dramatically upon digestion by proteases, resulting in a measurable indication of proteolysis.

Features

• Casein substrate: Measures activity of any protease that cleaves casein into peptide fragments
• Trypsin standard: Quantifies protease activity relative to trypsin, a universally accepted reference
• Sensitive: 1000 times more sensitive than assays using unmodified forms of casein
• Simple and fast: Test tube and microplate protocols completed in less than one hour
• Homogenous: Addition-only steps; no separation, transfer, or stop steps required
• Customizable: Easily adapt time, temperature, and pH to optimize sensitivity for proteases of interest

Applications

• Assess functional integrity and progress of protease purification
• Quantify protease contamination in protein samples
• Characterize activity of unusual proteases compared to trypsin
• Evaluate buffer conditions for effects on protease function and activity

Additional Specifications

• Substrate: Fluorescein-labeled casein (FITC-casein); available separately as Part No. 23267
• Basis of assay: Change in fluorescence resonance (FRET) or fluorescence polarization (FP)
• Measurement: Instrument with fluorescein excitation and emission filters (485/538 nm)

Related Products

• Pierce™ Colorimetric Protease Assay Kit (Cat. No. 23263)
• FITC-Casein for Pierce™ Fluorescent Protease Assay Kit (Cat. No. 23267)

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