Thermo Fisher Scientific MDM2 Monoclonal Antibody (SMP14)
MDM2 단백질을 인식하는 Mouse monoclonal antibody (Clone SMP14). Western blot, IHC, ELISA에 사용 가능. Human, Mouse, Rat 반응성. Protein A/G 정제, PBS(pH 7.4) buffer에 1 mg/mL 농도로 제공. 연구용 전용.
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- 4193-MSM1-P1ABX
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- pk
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Applications
| Application | Tested Dilution | Notes |
|---|---|---|
| Western Blot (WB) | 1–2 µg/mL | |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent | |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 1–2 µg/mL | |
| ELISA | Assay-dependent |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | SMP14 |
| Immunogen | Synthetic peptide (aa 154–167) of human MDM2 protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | −20°C, Avoid freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
MDM2 is a ubiquitin ligase for p53 and plays a central role in regulating the stability of p53. It is located on chromosome 12q. Akt-mediated phosphorylation of MDM2 at Ser166 and Ser186 enhances its interaction with p300, leading to ubiquitination and degradation of p53. Phosphorylation also blocks binding to p19ARF, further increasing p53 degradation. Overexpression of MDM2 can inactivate p53 and reduce its tumor suppressor function. MDM2 is implicated in apoptosis and cell cycle regulation and is overexpressed in various human malignancies, including soft tissue carcinomas and breast cancer. It interacts with proteins such as retinoblastoma 1 and ribosomal protein L5, and more than 40 alternatively spliced transcript variants have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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