Thermo Fisher Scientific Cycloxygenase-2 (COX-2) Recombinant Rabbit Monoclonal Antibody (COX2/3320R)

Applications

Immunohistochemistry (Paraffin) (IHC (P))

• Assay-dependent

Immunohistochemistry (PFA fixed) (IHC (PFA))

• 1–2 µg/mL

Product Specifications

Target Information

COX2 converts arachidonate to prostaglandin H2 (PGH2), a committed step in prostanoid synthesis, including production of inflammatory prostaglandins. The conversion of arachidonate to prostaglandin H2 is a two-step reaction:

1. A cyclooxygenase (COX) reaction converting arachidonate to prostaglandin G2 (PGG2).
2. A peroxidase reaction reducing PGG2 to prostaglandin H2 (PGH2).

COX2 is constitutively expressed in some tissues under physiological conditions (e.g., endothelium, kidney, brain) and is up-regulated under pathological conditions such as cancer and inflammation. Up-regulation is associated with increased cell adhesion, phenotypic changes, resistance to apoptosis, and tumor angiogenesis. In cancer cells, COX2 facilitates production of prostaglandin E2 (PGE2), which modulates motility, proliferation, and apoptosis resistance.
COX2 is naturally inhibited by calcitriol (active Vitamin D). Glucocorticoids repress PTGS2 gene activity by interfering with transcription initiation and elongation. COX2 is a known target of NSAIDs such as aspirin, which reduce pain and swelling from COX2-driven inflammation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.