Thermo Fisher Scientific HP1 alpha Polyclonal Antibody
Human CBX5 단백질에 특이적인 Rabbit Polyclonal 항체로, Western blot, IHC(P), ICC/IF에 사용 가능. 항원 친화 크로마토그래피로 정제되었으며, PBS(40% glycerol) 버퍼에 보관. HP1 alpha 단백질 연구에 적합.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.04–0.4 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:500 |
| Immunocytochemistry (ICC/IF) | 0.25–2 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein corresponding to Human CBX5. Recombinant protein control fragment (Product #RP-91590). |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.10 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage buffer | PBS, pH 7.2, with 40% glycerol |
| Contains | 0.02% sodium azide |
| Storage conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping conditions | Wet ice |
| RRID | AB_2789714 |
Product Specific Information
Immunogen sequence:
VVKGQVEYLL KWKGFSEEHN TWEPEKNLDC PELISEFMKK
YKKMKEGENN KPREKSESNK RKSNFSNSAD DIKSKKKREQ
SNDIARGFER GLEPEKIIGA TDSCGDLMTarget Information
Heterochromatin protein-1 (HP1) is a methyl-lysine binding protein localized at heterochromatin sites, where it mediates gene silencing. Mammalian methyltransferases that selectively methylate histone H3 on lysine-9 generate a binding site for HP1 proteins, which are adaptor molecules involved in gene silencing and chromatin structure. High-affinity recognition of methylated histone H3 by HP1 requires a functional chromodomain, making it a specific interaction motif for the methyl epitope on lysine-9. In vivo, heterochromatin association of HP1 proteins is lost in Suv39h double-primary mouse fibroblasts but restored after reintroduction of catalytically active SUV39H1 HMTase.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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