Thermo Fisher Scientific VetMAX-Gold BVDV PI Detection Kit

Applied Biosystems™ VetMAX™-Gold BVDV PI Detection Kit

The Bovine Virus Diarrhea RNA Test Kit (VetMAX™-Gold BVDV Detection Kit) is designed for rapid, in vitro detection of bovine viral diarrhea virus (BVDV RNA) extracted from ear notches in acutely or persistently infected cattle.

This kit is the first USDA-licensed real-time PCR assay for detecting BVDV in cattle. It performs a single-tube, real-time reverse transcription-polymerase chain reaction (RT-PCR), where RNA is reverse transcribed into cDNA and amplified using fluorescent TaqMan™ probes for real-time detection.

Key Features

• Proven repeatability and reproducibility in field studies
• Highly specific and sensitive detection of BVDV types 1 and 2
• Consistently detects fewer than 40 RNA copies
• Includes Xeno™ RNA internal positive control for result confirmation
• Rapid and cost-effective detection with results in approximately 1.5 hours
• Simple, fast workflow minimizes sample handling and cross-contamination
• Easily automated for high-throughput processing
• Detects BVDV in ear-notch tissue
• Utilizes robust AmpliTaq Gold™ DNA Polymerase and TaqMan™ technology

Limitations

• Not intended for viral subtype discrimination
• Samples must be handled properly to prevent RNA degradation
• RNA extraction methods should yield RNA free of RT-PCR inhibitors
• Follow good PCR practices to avoid false positives due to contamination

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