Thermo Fisher Scientific HES6 Polyclonal Antibody
HES6 단백질을 인식하는 Rabbit Polyclonal 항체로, Western blot, IHC, ICC/IF, ELISA 등에 사용 가능. Human, Mouse, Rat 반응성. Protein A 정제, 1 mg/mL 농도, -20°C 보관. 연구용으로만 사용.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:200 |
| Immunohistochemistry (Frozen) (IHC-F) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
| ELISA | 1:500–1:1,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH-conjugated synthetic peptide derived from human HES6, amino acids 1–80 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.01M TBS, pH 7.4, with 50% glycerol, 1% BSA |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
The HES family members form a complex with TLE, the mammalian homologue of Groucho, mediated by the carboxy-terminal WRPW motif of HES proteins. The HES/TLE complex binds directly to DNA rather than interfering with activator proteins. Most HES family members, including HES1 and HES5, preferentially bind to the N box (CACNAG) rather than the E box (CANNTG). HES2 binds both N and E box sites, while HES6 does not bind DNA but inhibits HES1 activity to promote transcription. HES1 and HES2 are expressed in various adult and embryonic tissues. HES3 is expressed in cerebellar Purkinje cells, and HES5 is found in the nervous system. HES6 is expressed in the brain and limb buds of developing embryos.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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