Thermo Fisher Scientific Bu-1b Monoclonal Antibody (5-11G2), PE
Thermo Fisher Scientific의 Bu-1b Monoclonal Antibody (5-11G2), PE는 닭 Bu-1b 항원을 검출하는 단클론 항체로, Flow Cytometry에 적합합니다. PE 형광표지로 높은 감도 제공, 4°C 암소 보관, 연구용으로만 사용됩니다.
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Applications
Flow Cytometry (Flow)
- Tested Dilution: 0.2 µg/10^6 cells
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Chicken |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 5-11G2 |
| Immunogen | Bursa cells from two- to five-week-old CHA (Bu-1b) strain chickens |
| Conjugate | PE (R-Phycoerythrin) |
| Excitation / Emission Max | 565 / 576 nm |
| Form | Liquid |
| Concentration | 0.1 mg/mL |
| Purification | Purified |
| Storage Buffer | PBS, pH 7.5, with proprietary stabilizer |
| Contains | <0.1% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2745717 |
Additional Formats
- Unconjugated ([MA5-28759])
- Biotin ([MA5-28756])
- FITC ([MA5-28757])
- Request custom conjugation (Thermo Fisher Custom Form)
Target Information
Bu-1b, a product of the Bu-1b allele, is expressed on the cell surface as a disulfide-linked homodimer. In 10-week-old H.B15 and H.B2 chickens, Bu-1 is found on:
- 85–90% of bursal cells
- 2–8% of thymocytes
- 15–27% of spleen cells
- 2–18% of peripheral blood cells
It is also expressed on subsets of macrophages and monocytes but not on granulocytes, erythrocytes, or thrombocytes.
In chickens heterozygous for the Bu-1 alleles (Bu-1a/b), Bu-1a does not exhibit allelic exclusion.
The monoclonal antibody 5-11G2 reacts with lymphoid cells from CHA and H.B14Ab strains but does not react with cells from H.B14Aa.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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