Thermo Fisher Scientific Thioredoxin 2 Polyclonal Antibody

Applications

• Western Blot (WB): 1:500–1:2,000

Product Specifications

Product Specific Information

• Purity is >95% by SDS-PAGE.

Target Information

Thioredoxins (Trx) are small, multi-functional proteins with oxidoreductase activity and are ubiquitous in essentially all living cells. Trx contains a redox-active disulfide/dithiol group within the conserved Cys-Gly-Pro-Cys active site. The two cysteine residues in the conserved active centers can be oxidized to form intramolecular disulfide bonds.
Reduction of the active site disulfide in oxidized Trx is catalyzed by Trx reductase with NADPH as the electron donor. The reduced Trx acts as a hydrogen donor for ribonucleotide reductase, an essential enzyme for DNA synthesis, and serves as a potent general protein disulfide reductase with numerous functions in growth and redox regulation.
Specific protein disulfide targets for reduction by Trx include protein disulfide-isomerase (PDI) and several transcription factors such as p53, NF-kB, and AP-1 (T1-151). Trx also contributes to H₂O₂ removal, particularly when coupled with methionine sulfoxide reductase or various peroxiredoxin isoforms.

Usage Note

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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