Thermo Fisher Scientific WT1 Monoclonal Antibody (7F9G7H2)
WT1 단백질을 표적하는 Mouse Monoclonal Antibody로 Western blot, Flow cytometry, ELISA에 사용 가능. Human 및 Non-human primate 반응성. Protein G로 정제된 1 mg/mL 액상 형태, 4°C 보관 권장. 연구용으로만 사용.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Flow Cytometry (Flow) | 1:200–1:400 |
| ELISA | 1:10,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Non-human primate |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 7F9G7H2 |
| Immunogen | Purified recombinant fragment of human WT1 (AA: 1–181) expressed in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long-term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_3090537 |
Target Information
The Wilms Tumor 1 (WT1) gene encodes one of the WPP domain-interacting proteins required for RanGAP nuclear envelope association in root tip cells.
Ran GTPase plays essential roles in nucleocytoplasmic transport, spindle formation, and postmitotic nuclear envelope reassembly.
The cytoplasmic Ran GTPase activating protein (RanGAP) is critical for establishing a functional RanGTP/RanGDP gradient across the nuclear envelope and is associated with the outer surface of the nuclear envelope in metazoan and higher plant cells.
Arabidopsis thaliana RanGAP association with the root tip nuclear envelope requires a family of likely plant-specific nucleoporins combining coiled-coil and transmembrane domains (CC-TMD) and WPP domain-interacting proteins (WIPs).
Wilms Tumor 1 and WIT2 have been identified as a second family of CC-TMD proteins, structurally similar yet distinct from the WIP family, required for RanGAP nuclear envelope association in root tip cells.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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