Thermo Fisher Scientific Phospho-Paxillin (Ser273) Polyclonal Antibody
인간 및 생쥐 Paxillin (Ser273) 인산화 형태를 검출하는 Rabbit Polyclonal 항체. Western blot에 최적화(1:500~1:3,000 희석). 항원 친화 크로마토그래피로 정제되어 높은 특이성과 재현성 제공. 연구용으로만 사용 가능.
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Applications
- Western Blot (WB): 1:500–1:3,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Carrier-protein conjugated synthetic peptide corresponding to residues around human Paxillin (phospho Ser273) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.04 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 1% BSA, 20% glycerol |
| Contains | 0.025% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2792859 |
Product Specific Information
- Keep as concentrated solution.
- Predicted reactivity: Mouse (100%), Rat (100%), Xenopus laevis (100%), Chicken (100%).
- Positive Control: C8D30, NIH-3T3, A431 (100 nM Calyculin A treatment for 30 min).
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Paxillin is a focal adhesion protein and a substrate for several tyrosine kinases such as src, FAK, and p210BRC/ABL. The tyrosine phosphorylation of paxillin is affected by conditions that change cell-cell adhesion. Paxillin associates tightly with FAK and Crk through its SH2 domain. This interaction is independent of the extracellular matrix. Although paxillin was initially discovered in fibroblasts, its phosphorylation may also be important during neurite extension during differentiation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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