Thermo Fisher Scientific CYB5R3 Monoclonal Antibody (OTI1E3), TrueMAB
인간 CYB5R3 단백질을 인식하는 단클론 항체로 Western blot, ICC/IF, Flow Cytometry 등에 사용 가능. 마우스 IgG1 아이소타입, 친화 크로마토그래피로 정제. 동결건조 형태로 제공되며, PBS 완충액(8% 트레할로스) 포함. 연구용으로만 사용.
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Applications and Tested Dilution
| Application | Tested Dilution | References |
|---|---|---|
| Western Blot (WB) | 1:500 | - |
| Immunocytochemistry (ICC/IF) | 1:100 | - |
| Flow Cytometry (Flow) | Assay-dependent | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI1E3 |
| Immunogen | Full length human recombinant protein of human CYB5R3 produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
Gene: CYB5R3 (NADH-cytochrome b5 reductase 3, diaphorase 1)
Protein Length: 301 amino acids
Family: Flavoprotein pyridine nucleotide cytochrome reductase family
CYB5R3 has two naturally occurring isoforms:
- Isoform 1: Anchored to the cytoplasmic side of the endoplasmic reticulum and outer mitochondrial membrane.
- Isoform 2: Soluble form found in erythrocytes.
Functions:
- Fatty acid desaturation and elongation
- Cholesterol biosynthesis
- Drug metabolism
- Methemoglobin reduction in erythrocytes
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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