Thermo Fisher Scientific ZO-1 Polyclonal Antibody
ZO-1 단백질을 인식하는 Rabbit Polyclonal Antibody로, WB 및 ICC/IF 실험에 적합합니다. 인간에 반응하며 고순도 항원 친화 크로마토그래피로 정제되었습니다. 세포 간 밀착연접 연구 및 암 관련 단백질 발현 분석에 활용됩니다.
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Thermo Fisher Scientific ZO-1 Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:3,000 |
| Immunocytochemistry (ICC/IF) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fragment corresponding to amino acids 1574–1748 of ZO-1 (Uniprot ID: Q07157) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS (pH 7) with 1% BSA, 20% glycerol |
| Contains | 0.01% thimerosal |
| Storage Conditions | Store at 4°C (short term). For long-term, store at -20°C and avoid freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_11153017 |
Product Specific Information
- Recommended positive controls: HCT116, 293T, A431, HeLa
- Predicted reactivity: Mouse (99%), Rat (99%), Dog (84%)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
ZO-1 forms complexes with ZO-2 or ZO-3 and associates with claudin, occludin, and F-actin at tight junction strands, linking the actin cytoskeleton to the junction.
ZO-1 expression is reduced in many breast cancer lines. ZO-2 and ZO-3 are ubiquitously expressed in epithelial tight junctions, while ZO-1 is also found at cardiac myocyte junctions.
ZO-1 may be involved in signal transduction at cell-cell junctions. Two transcript variants encoding distinct isoforms have been identified for the ZO-1 gene.
Diseases associated with ZO-1 dysfunction include Celiac Disease and Congenital Nephrotic Syndrome.
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm.
For more information, visit www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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